Preservation of Leukocytes in Conditions of Cryoanabiosis

Clinicians have long faced the problem of preserving cells and realize the need to maintain functionality of cells, which is possible under conditions of deep cryoanabiosis (cryopreservation) (−196°C). This method of preservation, is for various reasons, not accessible for wide application. The purpose of this work was to develop an effective, accessible, and economical method for preservation of functionally viable leukocytes at a moderately low temperature of −40°C. It was shown that an application of an original cold-protective solution and a nonlinear freezing program allows granulocytes to be kept in a functionally viable condition for 30 days. Furthermore, T-lymphocytes are the most stable cells while under the damaging influence of cryoanabiosis (−40°C).