ColabFold - Making protein folding accessible to all

Summary ColabFold is an easy-to-use Notebook based environment for fast and convenient protein structure predictions. Its structure prediction is powered by AlphaFold2 and RoseTTAFold combined with a fast multiple sequence alignment generation stage using MMseqs2. MMseqs2’s MSAs produce more accurate predictions while being ~16 faster compared to the AlphaFold2’s MSA stage. ColabFold also offers many advanced features, such as homo- and hetero-complex modeling and exposes AlphaFold2 internals. When coupled with Google Colab, ColabFold becomes a free and accessible platform for protein folding that does not require any installation or expensive hardware. Code ColabFold is free open-source (MIT) [1] software available at https://github.com/sokrypton/ColabFold. Contact milot.mirdita@mpibpc.mpg.de, so@fas.harvard.edu, martin.steinegger@snu.ac.kr