Isolation of DNA from HSV-2 and Purification of ICP10 Promoter Gene

Objective Amplification,purification of ICP10 promoter gene.Methods DNAof HSV-2 isisolated fromculture vero cells.Apair of PCRpri mers were created fromthe ICP10 promoter gene of HSV-2(Sal 5 strain) by DNAStar software andtworestrictionsites(Bgl Ⅱand HindⅢ) wereincluded as part of thepri mers.The genefragment codingfor ICP10 promoter was amplified by PCR.The PCRproduct was purified byPCRpurification kit.Results A641bpfragment was amplified by PCR.The gene fragment was provedto cor-rect by agarose gel electrophoresis.Conclusion The gene fragment is used to ligate to vector after DNAse-quencing.