[In vitro study on the anti-HSV-1 and HBV activities of extracts from the fruit of Eucalyptus maidenii].

Objective:To investigate the antiviral activities of three kinds of extracts from the fruit of Eucalyptus maidenii against herpes simplex virus type1 and Hepatitis B Virus.Methods:Cytotoxicity of extracts on Vero cell lines were estimated using MTT method and anti-HSV-1 activity was observed and determined with CPE and plaque reduction assay.The inhibitory effects of extracts on HBsAg and HBeAg secretion in HepG2.2.15 cell culture were detected using ELISA.Results:Aqueous extract(p18-E3)had conspicuous anti-HSV-1 activity,the IC50 was 126.77 μg/mL,but the EtOAc extracts(p18-E1)and MeOH extracts(p18-E2)showed little anti-HSV-1 activity.None of these extracts had significant inhibitory eflect on HBsAg and HBeAg secretion in HepG2.2.15 cell culture.Conclusion:Aqueous extract(p18-E3)from the fruit of Eucalyptus maidenii has conspicuous anti-HSV-1 activity.It could inactivate virus directly,and inhibit virus attachment,but had no influence on virus penetration.The mechanism that p18-E3 inactivates virus might involve in viral envelope alteration.