Increasing the ease and speed of eukaryotic protein expression: a new cell-free in vitro translation system based on Sf insect cell extracts

Background For researchers looking for fast access to their protein of interest, cell-free expression systems are an attractive option, as they do not require specialized equipment, are open systems, and offer fast screening of expression constructs for expression efficiency, yield, solubility and other criteria (e.g. requirement of cofactors). A broad range of eukaryotic proteins require posttranslational modifications such as phosphorylation, glycosylation, or signal peptide cleavage to display full functional activity.