Application of SELDI-TOF-MS technique in diagnosis of hepatocellular carcinoma

Objective To screen serum biomarkers in patients with hepatrometry(SELDI-TOF-MS)technique.Methods Proteinic spectra were generated by SELDI-TOF-MS.The sera from 60 unaffected healthy donors,40 patients with liver eaneer and 20 patients with hepatocirrhosis were analyzed for selection of the specific protein of liver cancer.The biomarker patterns were used. Results Five protein markers were highly expressed and one protein markers was lowly expressed in serum of liver cancer pa- tients.Two protein peaks(8943Da and 4477Da m/z)were used to set up diagnostie modelⅠfor differentiation of liver cancer pa- tients and healthy volunteers with a sensitivity of 90%(36/40)and specificity of 10%(60/60).The other three protein peaks (4477Da,13761Da and 4097Da m/z)were used to set up a diagnostic modelⅡfor differentiation of liver hepatoeeLllular earcinoma patients and hepatoeirrhosis diseases with a sensitivity and specificity of 85 %(34/40)and 90 %(18/20),respectively.Conclusion SELDI-TOF-MS offers a unique platform for the proteomie deteetion of hepatoeelllular carcinoma and it is also a noninvasive method for further study of the proteomic changes in the development and progression of liver cancer.