The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome
2016
To study the mechanisms involved in the maintenance of a linear
mitochondrial genome we investigated the biochemical properties
of the recombination protein Mgm101 from Candida parapsilosis.
We show that CpMgm101 complements defects associated with the
Saccharomyces cerevisiaemgm101–1ts mutation and that it is
present in both the nucleus and mitochondrial nucleoids of C.
parapsilosis. Unlike its S. cerevisiae counterpart, CpMgm101 is
associated with the entire nucleoid population and is able to
bind to a broad range of DNA substrates in a non-sequence
specific manner. CpMgm101 is also able to catalyze strand
annealing and D-loop formation. CpMgm101 forms a roughly
C-shaped trimer in solution according to SAXS. Electron
microscopy of a complex of CpMgm101 with a model mitochondrial
telomere revealed homogeneous, ring-shaped structures at the
telomeric single-stranded overhangs. The DNA-binding properties
of CpMgm101, together with its DNA recombination properties,
suggest that it can play a number of possible roles in the
replication of the mitochondrial genome and the maintenance of
its telomeres.
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