Isolation and Identification of High Cellulolytic Activity of Bacteria from Golden Snail’s (Pomacea canaliculata) gut

Cellulase can degrade cellulose to become glucose. A large number of cellulolytic microbes are available in the animal’s gut. Theaim of this research was to isolate and identify the cellulolytic bacteria with high activity from Golden snail’s gut. Some golden snailswere surface sterilized by 70% ethanol and then washed in sterile distilled water. The entire guts were removed from the abdomenand disrupted then 1 gram of gut debris was suspended in 10 ml sterile distilled water. After serial dilution, aliquots were transferredinto CMC-solid media and incubated at 35° C for 24 hours. The growing cultures were cultivated on NA-solid media. Bacteria fromsingle colonies were repeatedly grown on CMC-solid medium and covering the Petri dishes with Congo red dye. The colonies that hadhigh degradation ability then tested to endoglucanase activity and identifi ed with biochemical and 16S-rRNA tested. The result of thisresearch is there were two high cellulolytic bacteria isolates Burkholderia pseudomallei and Klebsiella sp.