Preparation method of liposome entrapping water-soluble medicines

The invention relates to a preparation method of a liposome entrapping water-soluble medicines. According to the invention, a water-soluble medicine is dissolved in amino-acid-structure-containing high-molecular hydrophilic colloids such as gelatin, collagen or albumin; poloxamer is added and the mixture is well mixed, such that a W/W type colloidal solution is formed; the solution is lyophilized; the formed lyophilized powder is transferred into a tert-butanol solution containing a liposome material, and the mixture is well dispersed; and the mixture is subjected to lyophilization, such that high-entrapment-efficiency liposome entrapping the water-soluble medicine is formed. According to the liposome preparation method, W/W type colloid is combined with a two-step lyophilization preparation technology, such that problems such as low water-soluble medicine load, low entrapment efficiency and poor stability of existing preparation methods of liposome entrapping water-soluble medicines are solved. The method provided by the invention is used for preparing liposome used for entrapping water-soluble medicines, and is especially suitable for preparing liposome used for entrapping poor-thermal-stability or macromolecular medicines. The method can also be used for covering medicine bitterness or odor, and for separating medicines from other components.