Количественные параметры клеток опухоли у больных пограничными новообразованиями яичников

Objective: to specify the prognostic value of DNA levels, their distribution by the phase of a cell cycle, as well as the proliferative activity index in borderline ovarian tumors (BOT). Subjects and methods. The study included 88 patients with BOT whose age was 16 to 80 years (mean age 42.4±1.4 years), 51 (57.9%) patients being under 45 years of age. A control group consisted of 18 patients with benign ovarian masses (BOM). An EPICS-XL (Coulter, USA) laser flow cytofluometer was used to study cellular DNA. The findings were analyzed, by applying the MultiCycle program (Phoenix Flow Systems, USA) employed to analyze ploidy and the count of tumor cells in each phase of a cell cycle. Results. Diploid and aneuploid tumors were found in 55 (62.5%) and 33 (27.5%) patients with BOT, respectively. Comparative analysis of DNAflow cytometric parameters in the BOT and BOM groups revealed statistically significant differences in DNA index (DNAi). Analysis of the distribution of tumors by age indicated that DNAi and the distribution of their cell cycle were age-unassociated. As the disease progressed, the patients with diploid tumors increased in number and those with aneuploid tumors decreased. At the same time these differences were statistically insignificant. The analysis of the distribution of a tumor cell cycle by the stage of the disease in patients with BOT did not reveal any significant differences in the compared groups either. Examining the distribution of tumor cells by the phases of a cell cycle showed that the rate of cell fission in serous BOTs was higher than that in mucinous BOTs. Conclusion. BOTs are a heterogeneous group more than half (61.9%) of them are aneuploid, in 42.4% of the patients, their number was in the range of 20-40%. No regularities were found in the distribution of the number of diploid BOTs in relation to the age of patients and to the stage of the disease.