Extraction of flavonoids from valerian optimized by response surface method

The extraction process of flavonoid compounds originated from Valeriana ofecnilis( produced from Guizhou province) was investigated using ethanol as the extraction solvent. Method: Four factors,including concentration of Ethanol,ratio of raw material to solvent,extracting temperature and time,were optimized by response surface method.The optimum conditions were as follows: the concentration of ethanol was 53.0%; ratio of material to solvent was 1 ∶ 29 g / m L; extraction temperature was 73 ℃ and extraction time was 26 h. Under the optimum conditions,the difference of between real value of 2.133% and theoretic value in rate of extraction was about 3.5%. Conclusion: Response surface method was a reliable tool to optimize the extraction of flavonoid compounds from Valeriana ofecnilis.