Histologia do intestino de Boophilus microplus (Canestrini, 1887) (Acari: lxodidae) Histology of the intestine of Boophilus microplus (Canestrini, 1887) (Acari:lxodidae)

Figura 1: Estrutura celular do epitelio intestinal do Boophilus microplus: (A), corte transversal de intestino medio, tricromico de Masson. (160x); (B), corte sagital de intestino medio, tricromico de Masson (400x); (D), corte sagital de intestino medio, H.E. (1 OOOx). (L*), luz intestinal; (MB), membrana basal; (GS), grânulos de secrecao; (CB), celulas basais; (CD), celulas digestivas; (CS2), celulas secretoras tipo dois; (HB), hemoglobina; (CBF), celula basofilica; (CS 1), celula secretora tipo um; (L), leucocitos; (MC), membrana celular; (N), nucleo. A metodologia empregada mostrou-se eficiente na classificacao e observacao dos tipos celulares do intestino do 8. microplus. As coloracoes empregadas foram adequadas quanto a diferenciacao das celulas, onde pode-se observar os constituintes celulares como nucleo, nucleolo, membranas, grânulos de digestao e grânulos de secrecao (Fig. 1: A, B e C). Trata-se, portanto, de uma tecnica viavel para o estudo do trato digestivo do 8. microp/us. Females of the tick 8oophilus microplus at various stages in theirengorgement to have their intestine studied histologically. Ticks were inoculated with 1 O% formaldehyde through the peritrematic plate and a nus. Gut caeca were removed and separated from other internai organs. Sections were stained with hematoxylin-eosin and Masson's triple. lt was possible to observe basal, basophilic, digestive, secretory 8 1 and 8 2 cells for light microscopy. The technique was suitable to study of the intestine of 8. microplus.