Bimetallic Pt–M (M = Cu, Ni, Pd, and Rh) nanoporous for H2O2 based amperometric biosensors

Abstract Hydrogen dioxide, H 2 O 2 , is an electrochemically active species and involved in many enzymatic reactions. Therefore, detection of H 2 O 2 is often used to quantify H 2 O 2 generated analyte in an enzymatic reaction for biosensing application. Platinum based catalysts are usually employed enhancing the oxidation of H 2 O 2 resulting in an improved biosensor. In this study, platinum based bimetallic catalysts including PtRh, PtPd, PtCu, and PtNi were examined for their sensitivity, selectivity to H 2 O 2 detection. Interference by ascorbic acid and uric acid at concentration of 15 mg/L and 85 mg/L, the highest physiological level, were investigated, respectively. Preparation and characterization of these bimetallic catalysts were undertaken including TEM and XRD studies. The electrochemical detections of H 2 O 2 by these bimetallic catalysts were assessed. Experimental results would aid in the advancement of bimetallic Pt based catalysts for H 2 O 2 detection in biosensing applications. Among these bimetallic catalysts, PtRh exhibited an enhanced sensitivity of 839.9 μA mM −1  cm −2 ( R 2  = 0.998) with a low 0.86% deviation in the presence of the AA and UA.