The Effect of Sonic wave on the Differentiation of Mesenchymal Stem Cells

Ligament-bone integration was important for ligament tissue reconstruction. Graft integration is required for successful reconstruction of the anterior cruciate ligament. We made a comparative study on the effect of silk scaffold and lyophilized collagen/HA substrate-containing silk scaffold(composite silk scaffold). As this result, proliferation, and bone-related marker expression in vitro were well observed in UC-MSC-seeded composite silk scaffolds compared to the cell-seeded silk scaffold. Further, silk and composite silk scaffolds were implanted into the knees of rabbits as artificial ligament, and they were harvested 24 weeks after implantation for assessing ligament-to-bone integration. It is thought that the lyophilized collagen/HA substrate is highly biocompatible in vitro and enhances wound healing in vivo.