백양꽃(Lycoris sanguinea Maxim. var. koreana (Nakai) Koyama) 인편조직배양에 의한 기내번식

In order to establish the effective in vitro propagation system for Lycori sanguinea Maxim. var. koreana (Nakai) Koyama, bulbscales were cultured on the MS medium with 2,4-D, NAA, IBA and BA. Bulbs were dissected into three parts according to bulb height(upper, middle and bottom), and allowed for a culture on the MS medium with 0.1 mg·L -1 2,4-D plus 1.0 mg·L -1 BA, 0.5 mg·L -1 NAA plus 1.0 mg·L -1 BA, and 3.0 mg·L -1 IBA plus 3.0 mg·L -1 BA. Regardless of plant hormone treatments, the bottom part of the bulb including the disk proved to be most effective in forming the callus and bulblets. The MS medium with 3.0 mg·L -1 IBA plus 3.0 mg·L -1 BA proved to be best effective in inducing bublets from the callus, where about 7～8 bulblets per explant were formed. Bulblet enlargement was best when 6% sucrose was added to the MS medium with 3.0 mg·L -1 IBA plus 3.0 mg·L -1 BA, compared to 3% or 9% sucrose.