On some reactive polymers and immobilized enzymes

Abstract New reactive polymer carriers with ionic or neutral hvdrophilic matrices have been synthesized. Effective were the copolymers of 3− or 4-isothiocyanato styrene and acrylic acid, copolymers of 1-vinyl-2-pyrrolidone and maleic anhydride or acrylic acid but also with acrylic acid/styrene modified with maleimide groups. As effective reactive carriers were also used polyethyleneimine, polyethyleneimine carbonic acids, poly-(allyl alcohol), polyvinylethers and various carriers of different polymer combinations containing poly(vinyl alcohol) in form of crosslinked gels, coated tubes and synthetic pulp. The ability of these individual carriers to bind enzymes was studied. Various Darameters which affect the immobilization reaction and the properties of the immobilized enzymes were investigated.