[Effects of the +83C/T polymorphism in apolipoprotein AI gene on the changes of serum biochemical traits of gluocse and lipid metabolism induced by high-carbohydrate diets].

Objective The aim of the present study was to investigate the possible effects of the +83C/T polymorphism in apolipoprotein AI gene(apoA1) on the changes of serum lipids,glucose,insulin and the homeostasis model assessment of insulin resistance(HOMA-IR) induced by a high-carbohydrate diet in healthyyouth.Methods Fifty-six participants were given a washout diet for 7 d,followed by a high-carbohydrate diet for 6 d.The washout diet contained 15% protein,31% fat and 54% carbohydrate.The high-carbohydrate diet contained 15% protein,15% fat and 70% carbohydrate.Twelve hour fasting venous blood was drawn on the mornings of the first,eighth and fourteenth days.Blood lipids,glucose and insulin were measured,and HOMA-IR was calculated.The genome DNA was extracted and the apoA1 +83C/T polymorphism was determined by polymerase chain reaction-based restriction fragment length polymorphism.Results Triglyceride and insulin were found significantly increased in the subjects with the CC genotype,but not in the T carriers after the high-carbohydrate diet.Significant decreases of total cholesterol and LDL-C and a significant increase of HDL-C were observed after the dietary intervention of the high-carbohydrate diet.Conclusion The triglyceride and insulin changes after the high-carbohydrate diet can be modulated by the apoA1 +83C/T polymorphism,and the T allele may eliminate the increase in triglyceride and insulin induced by the high-carbohydrate diet.