Cell movements controlled by the Notch signalling cascade during foregut development in Drosophila
2004
Notch signalling is an evolutionarily conserved cell interaction mechanism,
the role of which in controlling cell fate choices has been studied
extensively. Recent studies in both vertebrates and invertebrates revealed
additional functions of Notch in proliferation and apoptotic events. We
provide evidence for an essential role of the Notch signalling pathway during
morphogenetic cell movements required for the formation of the
foregut-associated proventriculus organ in the Drosophila embryo. We
demonstrate that the activation of the Notch receptor occurs in two rows of
boundary cells in the proventriculus primordium. The boundary cells delimit a
population of foregut epithelial cells that invaginate into the endodermal
midgut layer during proventriculus morphogenesis. Notch receptor activation
requires the expression of its ligand Delta in the invaginating cells and
apical Notch receptor localisation in the boundary cells. We further show that
the movement of the proventricular cells is dependent on the short
stop gene that encodes the Drosophila plectin homolog of
vertebrates and is a cytoskeletal linker protein of the spectraplakin
superfamily. short stop is transcriptionally activated in response to
the Notch signalling pathway in boundary cells and we demonstrate that the
localisation of the Notch receptor and Notch signalling activity depend on
short stop activity. Our results provide a novel link between the
Notch signalling pathway and cytoskeletal reorganisation controlling cell
movement during the development of foregut-associated organs.
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