A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells

Harrison J. Wensley,David A. Johnston,Wendy S. Smith,Suzanne E. Holmes,Sopsamorn U. Flavell,David J. Flavell

A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells

2020

Harrison J. Wensley
David A. Johnston
Wendy S. Smith
Suzanne E. Holmes
Sopsamorn U. Flavell
David J. Flavell

Purpose The aim of this work was to develop a quantitative, flow cytometric method for tracking the endolysosomal escape of a fluorescently labelled saporin toxin.

Keywords:

Cell biology
Cytosol
Biochemistry
Flow cytometry
Endosome
Chemistry
Immunotoxin
Saporin
Toxin
Endocytic cycle
Biophysics
Cytotoxicity
Confocal microscopy

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