Processes contributing to the improvement in antifungal effect of bio-molecules extracted from Moroccan Laurus nobilis against Verticillium dahliae

This work offers comparisons between stakeholder’s labor works before and during extraction of biomolecules extracted from Laurus nobilis in Morocco. The objective of this study was to determine some paths to follow in order to have a better extract that can inhibit or minimize the development of Ver-ticilium dahliae . For essential oils, we studied the effect of harvest region, harvest period, plant part, drying temperature, pressure of distillation, distil-lation temperature and fraction of applied essential oil. For extracts with organic solvents, we compared their effect by pursuing both techniques dur-ing extraction. The first one was about using an organic solvent for total ex-traction; while the second technique concerned a fractional extraction by using some solvents and pursuing an increasing polarity. The results showed that the degree of development inhibition in plant pathogen show a signifi-cant difference. Thus, these findings will be helpful in preparing a technical draft to be followed in the extraction of biomolecules from this plant in or-der to obtain better results in getting antifungal compounds.