The effect of insulin on the changes in acid phosphatase activity and cAMP level in the primary monolayer culture of hepatocytes from newborn rats during anoxia

: Effects of insulin was studied in the monolayer hepatocyte cultures of new born rats under anoxic conditions. Insulin 10(-7) M stabilized lysosomal membranes obtained after 20 min anoxia. I hr incubation of hepatocytes with insulin in normal conditions, which was followed by anoxia, caused a significant increase of acid phosphatase activity in the fraction enriched with lysosomes. Insulin caused more distinct stabilization effect on lysosomal membranes when its action was prolonged. On the other hand, insulin caused more than 2-fold increase of cAMP content in hepatocytes within 2 min of exposition as compared with control cultures. When exposition of the cells with insulin exceeded 2 min, lowering of cAMP content was observed. These data appear to indicate that stabilizing effect of insulin was secondary towards cAMP level increase. Indirect connection between cAMP level, insulin stabilizing action and the state of lysosomal membranes were noted in the rat hepatocyte culture.