Role of Lipopolysaccharide and Nonspecific Porins of Yersinia pseudotuberculosis in the Reception of Pseudotuberculous Diagnostic Bacteriophage

The role of the outer membrane components of Yersinia pseudotuberculosis and Yersinia pestis in the adsorption of pseudotuberculous diagnostic bacteriophage (PDB) was assessed with two model bacteriophage-antigen systems, in which the target antigens were either in the solution or on the surface of polystyrene microspheres. It was established that the nonspecific OmpF and OmpC porins of Y. pseudotuberculosis, along with lipopolysaccharide, are involved in the reception of PDB. It was determined that molecular conformation of the porins plays a significant role in interaction with the bacteriophage. It was found that the O antigen of Y. pseudotuberculosis not only does not participate in the reception of PDB but also prevents it by shielding the receptor site on the Yersinia lipopolysaccharide core, which remains completely accessible for the bacteriophage on Y. pestis cells.