Restriction fragment length polymorphism in MC3R and MC4R genes and their association with carcass traits in chicken.

The SNPs of MC3R and MC4R genes in Jining Bairi chicken,Wenshang Luhua chicken and Laiwu Black chicken were detected by PCR-RFLP method.And the correlation between the polymorphism loci and the carcass traits were analyzed.As a result,an A/G mutation at nt 1 424 in MC3R gene and a G/T mutation at nt 923 in MC4R gene were identified,and the corresponding detection systems of Dde Ⅰ and FbrⅠwere established.The statistical analysis showed that the MC3R polymorphism locus had a significant association with live weight (P=0.041 8) and breast muscle weight (P=0.012 6).The MC4R polymorphism locus had a significant association with intermuscular fat width (P=0.036 6).The multiple-range test indicated that in MC3R polymorphism locus,the LSM of live body weight,carcass weight,semi-eviscerated weight,eviscerated weight and breast muscle weight of MN chickens were significantly higher than MM chickens or NN chickens (P 0.05).In MC4R polymorphism locus,the percentage of LSM of carcass and intermuscular fat width of AB chickens were significantly higher than BB chickens (P 0.05).