Relationship of high mobility group A2 protein and vascular endothelial growth factor-C expression with Iymph node metastasis in non-small cell lung cancer

Objective To investigate the relationship of high mobility group A2 protein (HMGA2)and vascular endothelial growth factor-C (VEGF-C) expressions with lymph node metastasis in non-small cell lung cancer (NSCLC).Methods Total RNA was extracted in NSCLC group and normal control group and then reversely transcribed to cDNA.HMGA2 and VEGF-C expressions were assayed by realtime quantitative polymerase chain reaction.Results HMGA2 and VEGF-C expressions had significant difference between NSCLC group and normal control group ( P ＜ 0.05).HMGA2 and VEGF-C expressions in NSCLC patients with lymph node metastasis were significantly higher than those in patients without lymph node metastasis ( P ＜0.05).Conclusions This study suggests that HMGA2 and VEGF-C are highly expressed in NSCLC tissues,which may be correlated with metastasis of lung cancer.Combined detection of HMGA2 and VEGF-C is of value in assessing the prognosis of NSCLC and provides a new method for targeted therapy of NSCLC. Key words: High mobility group A2 protein; Vascular endothelial growth factor-C; Non-small cell lung cancer; Real-time quantitative polymerase chain reaction