CONSTITUENTS FROM TAIWANESE SARCOPYRAMIS NEPALENSIS

Six flavonoids, quercetin (1), quercetin-3-O-α-L-rhamnoside (2), quercetin-3-O-β-D-glucoside (3), isorhamnetin-3-O-β-D-glucoside (4), kaempferol-3-O-β-D-glucoside (5), and rutoside (6), one triterpene, (+)-α-onocerin (7), three pheophorbides, pheophorbide A methyl ester (8), methyl (10S)-hydroxypheophorbide A (9), and (10S)-hydroxypheophorbide A (10), two steroids, β-sitosterol (11), stigmasterol (12), two benzenes, p-coumaric acid (13) and caffeic acid (14), along with three fatty acids, myristic acid (15), palmitic acid (16), and linolenic acid (17) have been isolated from the aerial of Sarcopyramis nepalensis Wall. Structures of 1-17 were elucidated by spectroscopic methods, including NMR, UV, IR, and MS techniques. Biological evaluation revealed that compounds 8-10 possessed significant cytotoxicity against KB (ED50 = 3.8-5.2 μg/mL) and Hela (ED50 = 3.4-4.2 μg/mL) human tumor cells. In addition, compounds 1, 2, 4, and 14 were proved to have potent anti-oxidative activity (ED50 = 5.92-10.49 μM) by DPPH scavenging test.