Cellular Engineering Mechanical and dimensional adaptation of rabbit carotid artery cultured in vitro

The effects of the mechanical environment on arterial walls were investigated in rabbit common carotid arteries, cultured for six days under three different intraluminal pressures (0, 80 and 160mmHg) in a per'fusion culture system. The mechanical responses following the culture were examined using a quasi-static pressure--diameter test. Specimen viability was determined by smooth muscle contraction induced with KCI. Eighteen out of 21 cultured segments showed a peak reduction in diameter of more than 10% and were used for the analysis. The arterial segments cultured at OmmHg had a significantly smaller diameter than those cultured at other pressures. The segments cultured at higher pressure had lower incremental elastic moduli at 20 and 80mmHg and higher moduli at 160mmHg. The walls of the cultured segments were thicker in groups with higher pressure. These results indicate that, even in culture, the mechanical environment is a major determinant for the mechanical property and dimensions of the arterial wall. Arterial walls may respond to their mechanical environment even if other factors, such as hormonal environment and nervous stimuli, are kept unchanged.