Preferential Vδ1 expression among TcR γ/δ-bearing T cells in human oral epithelium

The oral cavity is a septic area colonized by various bacterial species, and the oral mucosa is frequently submitted to microtraumas. Several mechanisms are implicated in the defence of the oral tissue, but little is known concerning the eventual presence and role of γ/δ T cells at this site. Samples of healthy keratinized oral mucosa were examined with immunochemica! techniques using anti-CD3, CD4, CD8, CD22, TcRδ1, Vδl and Vδ2 monoclonal antibodies. Whatever the site examined. γ/δ T cells represent at most 2% of the T-cell population, a value similar to that found in other tissues. In the connective tissue, under the basement membrane, Vδ2+γ/δ T cells are predominant whereas the epithelium mostly contains Vδ+γ/δ T cells. The significance of this preferential Vδ1 intraepithelial presence is discussed.