Expression of Transcripts Corresponding to Cluster Hs.633957 in Human Healthy and Tumor Tissues

Using computational methods of database analysis, we identified nucleotide sequences expressed predominantly in human tumors. Experimental evidence was obtained for one of these sequences, belonging to UniGene cluster Hs.633957 and located near the telomere of chromosome 7p22.3. The RNA sequences encoded by Hs.633957 encode no proteins, and their role in the human body is unknown. The expression pattern of the locus arouses fundamental and practical interest. We studied the expression of the Hs.633957 nucleotide sequence in normal and tumor human tissues by RT-PCR. In normal tissues of an adult human, a relatively low expression rate of this locus was observed only in heart and liver. In an embryo, expression was found in brain and kidneys. The Hs.633957 locus is expressed in tumors of various organs, including lung, intestines, breast, stomach, cervix, lymph nodes, and others. Thus, Hs.633957 is expressed predominantly in tumors and therefore may be considered a promising tumor marker.