Influence of Rho-associated Kinase Inhibitor Y-27632 on the Early Embryonic Development of Mouse

[Objective] The aim of the study was to examine the effect of Rho-associated kinase inhibitor Y-27632 on the early embryonic development of mouse.[Method] 2-cells from mouse were taken,after 1-2 days culturing in vitro,cell count and receptor transplantation and the observation on the influence of Rho-associated kinase inhibitor Y-27632 on cell count and the efficiency of embryo implantation were carried out.[Result] When the early embryo was treated by Y-27632,embryonic development and blastular prolapse from the zona pellucida as blastocyst cavity appears were delayed.The embryonic morula and blastular developmental rate of the experimental group were higher than those of control group.Compared with the same period of embryo,cell number was increased,the efficiency of implantation was proper.[Conclusion] Rho-associated kinase inhibitor Y-27632 played an important role in increasing early embryonic cell number and delaying embryonic development of mouse.