Bacillus oryzisoli sp. nov., isolated from rice rhizosphere
2016
The taxonomy of strain 1DS3-10T, a Gram-staining-positive, endospore-forming bacterium isolated from rice rhizosphere, was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel strain was grouped with established members of the genus
Bacillus
and appeared to be closely related to the type strains
Bacillus benzoevorans
DSM 5391T (97.9 %),
Bacillus circulans
DSM 11T (97.7 %),
Bacillus novalis
JCM 21709T (97.3 %),
Bacillus soli
JCM 21710T (97.3 %),
Bacillus oceanisediminis
CGMCC 1.10115T (97.3 %) and
Bacillusnealsonii
FO-92T (97.1 %). The fatty acid profile of strain 1DS3-10T, which showed a predominance of iso-C15 : 0 and anteiso-C15 : 0, supported the allocation of the strain to the genus
Bacillus
. The predominant menaquinone was MK-7 (100 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminolipids. Cell-wall peptidoglycan contained meso-diaminopimelic acid. DNA–DNA hybridization values between strain 1DS3-10T and the type strains of closely related species were 25–33 %, which supported that 1DS3-10T represented a novel species in the genus
Bacillus
. The results of some physiological and biochemical tests also allowed the phenotypic differentiation of strain 1DS3-10T from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 1DS3-10T represents a novel species of the genus
Bacillus
, for which the name Bacillus oryzisoli sp. nov. is proposed. The type strain of the novel species is 1DS3-10T (=ACCC 19781T=DSM 29761T).
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