Characterization of clones derived from HeLa1B and expressing ScRad52

Copyright information: Taken from "Potentiation of gene targeting in human cells by expression of Rad52"Nucleic Acids Research 2005;33(14):4639-4648.Published online 16 Aug 2005PMCID:PMC1187822.© The Author 2005. Published by Oxford University Press. All rights reserved () Detection of ScRad52 expression by semi-nested RT–PCR. All selected clones, except parental HeLa.1B, express the transgene. pMSGY52 DNA was used for PCR marker and GAPDH was used as RT–PCR control. () Cell survival after X-irradiation. Cells were irradiated at the indicated dosages and colonies were stained and counted after 10 days. Relative survival was calculated by dividing the number of colonies by the number of seeded cells. Each point represents the mean value ± SD of three independent experiments performed in triplicate and is shown on a logarithmic scale.