Cribage procedure using zebrafish and the blood-brain barrier.

Screening method for a substance with biological activity in the central nervous system (CNS), brain or eye, the method comprising: administering a test substance to test zebrafish determining effect of the test substance on the activity or function of the central, brain or eye, or effect of the test substance on a symptom or a disease or test fish central nervous, brain or eye system disorder, thereby identifying a substance with said biological activity nervous system; where it is known that the test substance crosses the blood brain barrier and the method is performed on zebrafish of any age; or known to the test substance does not cross the blood brain barrier and the method is performed on zebrafish of age less than five days post-fertilization for molecules of 960 daltons or greater, less than ten days post-fertilization for molecules of less 960 daltons not ejected by p-glycoprotein (pgp) or less than eight days post-fertilization for expelled molecules actively by pgp, and / or is performed on zebrafish of age at least five days post-fertilization for molecules of 960 daltons or more, or at least ten days post-fertilization for molecules of less than 960 daltons not expelled by pgp and at least eight days post-fertilization for actively expelled by pgp molecules, and comprises direct delivery of the test substance to bypass the blood brain barrier; or, without knowing whether the test substance may or may not cross the blood brain barrier, the method is performed in a zebrafish of age at least five days post-fertilization for molecules of 960 daltons or greater, and at least ten days fertilization for molecules of less than 960 daltons, with direct delivery of the test substance to bypass the blood brain barrier; where the stated ages refer Alpez zebra bred under standard conditions and are adjusted if the zebrafish are grown under conditions that accelerate or retard achievement of the equivalent stage of development.