Effect of heparin on expressions of cerebral Bcl-2 and Bax and its neuroprotective effect after cardiopulmonary resuscitation in rats

Objective To observe the effect of heparin on the expressions of cerebral Bcl-2 and Bax and its neuroprotective effects at 24h after cardiopulmonary resuscitation(CPR) in rats. MethodsThirty male SD rats were equally randomized into three groups of A (CPR with adrenaline 0.01mg/kg),H(CPR with adrenaline 0.01mg/kg and heparin 0.5mg/kg) and C(sham operated).Tracheal intubation and femoral artery/vein catheterization were performed after anesthesia.The cardiac arrest models were established in groups of A and B by asphyxia for 8min,which was followed by CPR.Neurological deficit(ND) scores were evaluated before anesthesia and at 24h after return of spontaneous circulation.At 24h after recovery,the brain was removed and the expressions of Bcl-2 and Bax in the cerebral cortex and hippocampus were detected with immunohistochemical straining. ResultsCompared with group A,ND scores were significantly decreased in group H(P0.05).Compared with group C,Bcl-2 expression in group H and Bax expression in group A were significantly increased(P0.01). Conclusion Heparin can attenuate brain damage by increasing Bcl-2 and reducing Bax expression.