Expression pattern analysis of terpene synthases of endangered Aquilaria sinensis under different stresses

Objective To analyze the expression of terpene synthases of Aquilaria sinensis,an endangered south medicine,and to predict the influences of the environmental factors and stresses on the synthesis of terpene in A.sinensis.Methods Two-year old seedlings and calli were treated by different stresses.The gene expression patterns of terpene synthases were analyzed by real-time qPCR.Results Wounding and cauterizing could induce the transcriptional expression of terpene synthases in the stems,and the effect of cauterizing was more significant.Low temperature inhibited the transcription of terpene synthases.In calli,methyl jasmonate(MeJA) treatment had the most effective induction.Conclusion Both wounding and cauterizing could induce the synthesis of terpenes in stems,while the cauterizing treatment might have better effect.Low temperature has negative influence on agarwood formation.For calli,different treatments could induce the terpene synthesis,while MeJA has the best efficacy.