Differential Expression of Tripartite Motif-Containing Family in Normal Human Dermal Fibroblasts in Response to Porcine Endogenous Retrovirus Infection (porcineendogenousretrovirus/tripartitemotif-containingfamily/lipopolysaccharide/xenotransplantation /oligonucleotidemicroarray)

Antiretroviral restriction factors may play an essential role in the safety of xenotransplantation. Therefore, the present study focused on investigation of the changes in the tripartite motif-containing fam- ily (TRIM) gene expression in normal human dermal fibroblasts with and without lipopolysaccharide sti- mulation in response to porcine endogenous retrovi- rus infection. Analysis of the expression profile of TRIMs was performed using oligonucleotide micro- arrays and QRT-PCR. Nine (TRIM1, TRIM2, TRIM5, TRIM14, TRIM16, TRIM18, TRIM22, TRIM27 and TRIM31) statistically significantly differentially ex- pressed genes were found (P < 0.05, one-way ANOVA). In conclusion, comprehensive analysis of retroviral restriction factor gene expression in human dermal fibroblasts before and after porcine endogenous ret- rovirus infection with and without LPS stimulation may suggest association of the selected TRIMs with antiretroviral activity.