Analytical Method Development and Validation for Simultaneous Estimation of Trifluoperazine, Chlordiazepoxide and Trihexiphenidyl in its Pharmaceutical Dosage form by RP-HPLC

A simple, rapid, economical, precise and accurate Reverse phase high performance liquid chromatographic (RPHPLC) method for simultaneous estimation of Chlordiazepoxide, Trihexyphenidyl HCl and Trifluoperazine HCl in Their Combined Dosage Form has been developed. The RP-HPLC method was developed for the simultaneous estimation of Chlordiazepoxide, Trihexyphenidyl HCl and Trifluoperazine HCl in their Combined Dosage Form development method has been achieved. The separation was attained by Column LC- 2010 AT C18 (250mm x 4.6 mm x 5 µm) and Buffer (pH 3.5) : Acetonitrile :TEA (80:20:0.1 v/v/v) as mobile phase, at a flow rate of 1 ml/min. Detection was carried out at Wavelength of 228 nm. Retention time of Chlordiazepoxide, Trihexyphenidyl HCl and Trifluoperazine HCl were found to be 3.807 min, 6.887 min and 4.667 min respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for Chlordiazepoxide 5-15 μg/ml, for Trifluoperazine HCl 0.5-1.5 μg/ml and for Trihexyphenidyl HCl 1-3 μg/ml. The Percentage recoveries obtained for Chlordiazepoxide, Trifluoperazine HCl and Trihexyphenidyl HCl were found to be in range of 99.27 ± 1.09, 99.57 ± 0.56 and 99.22 ± 0.51 respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of Chlordiazepoxide, Trihexyphenidyl HCl and Trifluoperazine HCl in their combined dosage form.