Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports

Recombinant β-1,4-galactosyltransferase (β1,4-GalT) and α-2,6-sialyltransferase (α2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu5Acα(2→6)Galβ(1→4)GlcNAcβ-O-(CH2) 6-NH2, on a water-soluble primer having GlcNAc residues through a α-chymotrypsin-sensitive linker.