[Effect of hydrogen sulfide on the expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells with Helicobacter pylori infection].

Objective: To investigate the effect of hydrogen sulfide(H2 S) on the expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells with Helicobacter pylori(H. pylori) infection and to explore its mechanism on gastric mucosa inflammation caused by H. pylori. Methods: GES-1 cells were cultured for 24 h and divided into a control group(neither H. pylori nor NaHS), an H. pylori group, a NaHS group(which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS), and H. pylori + NaHS group(which was further divided into 4 groups at 50, 100, 200, or 400 μmol/L NaHS). Each group was then cultured for 3, 6, or 12 h. The expression of CSE, NF-κB, and IL-8 mRNA was measured by RT-PCR, and their correlation was analyzed. Results: The expression of CSE, NF-κB, and IL-8 mRNA in GES-1 cells in the H. pylori group was higher than that in the control group. The expression of CSE in the 200 μmol/L NaHS group and 400 μmol/L NaHS group was lower than that of the control group(P0.05), whereas the expression of NF-κB and IL-8 in all NaHS groups had no statistical differences compared with the control group(P0.05). The expression of CSE, NF-κB, and IL-8 mRNA in all groups of NaHS, H. pylori + 200 μmol/L NaHS group, and H. pylori + 400 μmol/L NaHS group was lower than that in the H. pylori group(P0.05). There was positive correlation among the expressions of CSE, NF-κB, and IL-8 mRNA in the H. pylori group, the H. pylori + 200 μmol/L NaHS group, and the H. pylori + 400 μmol/L NaHS group(P0.05). Conclusion: H. pylori can induce NF-κB and IL-8 mRNA expression and upregulate CSE mRNA expression. At 200 and 400 μmol/L, NaHS can suppress H. pylori-induced NF-κB and IL-8 mRNA expression and ameliorate the morphology of H. pylori-induced GES-1 injury, which may protect gastric epithelial cells by H. pylori infection.