Chiral Separation of Enantiomers of Four Amino Acids and Two Chial Drugs through Capillary Electrophoresis

Enantiomers of amino acids and chiral drugs were separated by capillary zone electrophoresis using double(6-O-β-carboxy-methyl-1,4-maleic acid ester)-β-cyclodextrin(DOCB-β-CD) as chiral selector.The buffer solution used was 20 mmol/L phosphate.The influences of DOCB-β-CD concentration,background buffer pH value,temperature and applied voltage were investigated.Under the respective optimum condition,enantiomers separation of four kinds of DL-amino acids(Phenylalanine,Tryptophan,Tyrosine and Histidine) and two kinds of chiral drugs(rofecoxib and rosiglitazone) were attained with a clear separation from the baseline.