Ultrasensitive Visualization of Virus via Explosive Catalysis of Enzyme Muster Triggering Gold-Nanoaggregate Disassembly

Sensitive and accurate diagnosis of viral infection is important for human health and social safety. Herein, by means of explosive catalysis from enzyme muster, a powerful naked-eye readout platform has been successfully constructed for ultrasensitive immunoassay of viral entity. Liposome was used to encapsulate multiple enzymes into an active unit. And its triggered rupture could boost the disassembly of gold-nanoaggregates that were crosslinked by peptides with opposite charges. As a result, plasmonically colorimetric signals were rapidly generated for naked-eye observation. Further harnessing the immunocapture, enterovirus 71 (EV71), a class of highly infective virus, was sensitively assayed with a detection limit down to 16 copies/μL. It is superior to the single enzyme anchored immunoassay system. Most importantly, the colorimetric assay was demonstrated with 100% clinical accuracy, displaying strong anti-interference capability. It is expectable that this sensitive, accurate, convenient strategy cou...