Effects of dietary supplementation of a mixture of ferulic acid and probiotics on the fillet quality of Megalobrama amblycephala fed with oxidized oil

Abstract To investigate the effects of feed composition on the quality of fillets, Megalobrama amblycephala were fed three diets containing either soybean oil (S group) or oxidized soybean oil (OS group), and oxidized soybean oil supplemented a mixture of ferulic acid and probiotics (BS group) for 8 weeks. After the feeding trial, fish fillets were collected for determining flavor nucleotides and free amino acids (FAAs). In addition, gas chromatography-ion migration spectrometry (GC-IMS) was used to analyze the volatile components and flavor profile in the fillets. The results showed that oxidized soybean oil contributed to the accumulation of off-flavor nucleotides such as hypoxanthine (Hx), hypoxanthin riboside (HxR), and umami-related FAAs. Mixtures of ferulic acid and probiotics promoted the accumulation of umami nucleotides, such as guanosine monophosphate (GMP) and inosine monophosphate (IMP), and off-flavor FAAs. The content of GMP and IMP in BS group was significantly (P