Mapping QTLs Conferring Salt Tolerance During Germination in Solanum pimpinellifolium Using AB-QTL

An advanced back-cross BC3S1 population consisted of 280 individuals was constructed from the cross between Solanum lycopersicum 9706 and Solanum pimpinellifolium LA722 after large scale screening under salt stress during seed germination of each backcross family.One hundred and thirty one polymorphic markers including 26 CAPS,66 SSR and 39 SRAP were used to construct a linkage map.Using germination index of 20,24 and 28 d to detected QTLs respectively,and it confirmed that germination index of 24 d is the optimum index.A total of 11 favourable QTLs of salt tolerance were detected using MQM mapping methods,of which 8 QTLs were derived from salt-tolerant wild parents LA722.All significant QTLs accounted for 73.2% of the totlal phenotypic variation.Fifty eight families with high salt tolerance and favorable economic traits were obtained.Family 230-1 and 230-3 contained qST-8-1 locus,family 228-4 contained qST-9-2 locus,while 231-6,210-7,226-1,226-6,226-7,204-7 contained both QTLs.These families had similar agronomic traits with 9706 and could be used as breeding materials.