UNILOCULAR FAT CELLS IN THREE-DIMENSIONAL COLLAGEN GEL MATRIX CULTURE

Summary Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. M These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture. - Sugihara, H., N. Yonemitsu, S. Tda, S. Miyabara, S. Funntsumaru, and T. Matsumoto. Unilocular fat cells in three-dimensional collagen gel matrix culture.J. Lipid Res. 1988. 29: 691-697.