[Mechanism of action of butylphalide against the injury following oxygen glucose deprivation/reoxygenation in rat cortical neurons].

To explore the mechanism of action of butylphalide(NBP) against the injury following oxygen glucose deprivation/reoxygenation(OGD/R) in rat cortical neurons,neurons of Wistar newborn rats were prepared by filtering through a mesh,centrifugation and trypsogen digestion.A simple,stable and reliable in vitro model of OGD/R of neurons was established.We studied the activation,the nuclear translocation of(NF-κB) p65 and the mRNA expression of iNOS affected by NBP in each group neuron by RT-PCR.NBP is proved to be able to add cellular vigor and decrease LDH release.The mRNA expression of iNOS in neurons after OGD 4 h/R 8 h decreased when treated with NBP.There is statistical difference between each concentration of NBP that it adds cellular vigor,decreases LDH release and expression of iNOS in neurons after OGD 4 h/R 8 h.There is also statistical difference between NBP(100 μmol·L~(-1)) and PDTC(100 μmol·L~(-1)).It is proved that NBP can protect neurons,block upregulation of iNOS mRNA,and restrain activation of NF-κB in neurons.