Direct Detection of (1-3)-β-Glucanase Isozymes in Isoelectrofocusing Gels Using a Dye-Labeled Substrate

1994

A procedure for the direct detection of (1-3)--glucanase isozymes in electrophoresis gels was developed. The procedure employed the commercial preparation of AZCL-pachyman as a chromogenic substrate for (1-3)--glucanases. The procedure detected the three basic isozymes which have been known to be expressed in germinating barley kernels. A major acidic and a minor isozymes were also detected in germinating kernels. The procedure was proved to be fast, simple and sensitive enough to be used for the analysis of the expression of (1-3)--glucanase isozymes in plant tissues. The detection limit of the procedure for the commercial preparation of Penicillium (1-3)--glucanase was estimated to be as low as 50U. The procedure could be used for the investigation of (1-3)--glucanases in laboratories facilitated with ordinary equipments and research personnel.

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