Inhibition Effect of Antagonistic Fungal Strain BOS-013 against the Pathogen of Rice Blast (Pyricularia oryzae)

[Objective] To search for the beneficial microorganisms and their metabolic products with control effect against rice blast,the paper studied the inhibition effect of the fermentation broth of actinomycetes BOS-013 strain against the pathogen of rice blast(Pyricularia oryzae).[Method] With the pathogen of rice blast as the target indicator fungus,the inhibition effect of the fermentation broth of BOS-013 strain and its extracts from different solvents against the pathogen were determined using cylinder plate method and filter paper method.[Result] The results showed that the inhibition rate of the fermentation broth of BOS-013 strain with fungus amount of 4×108 cfu/ml against the pathogen of rice blast was 92.0%±0.5%;the fermentation broth still had inhibition effect against the pathogen of rice blast after inactivation under high temperature and high pressure,and the inhibition rate was 60.0%±0.5%.After absorbed by non-polar CAD-45 type macroporous adsorption resin and eluted by 60% ethanol,the antifungal activity of the fermentation broth was still high,and the diameter of inhibition zone reached 33.0 mm.[Conclusion] The fermentation broth of BOS-013 strain had significant inhibition effect against the pathogen of rice blast.The results would provide foundation for further clarification of inhibition mechanism of BOS-013 strain fermentation broth against the pathogen of rice blast and its practical application.