Use of Nanoparticles as Nanoelectrodes in Contact-Less Cell Membrane Permeabilization by Time-Varying Magnetic Field: A Computational Study

This paper describes a computational approach for the assessment of electric field enhancement by using highly conductive gold nanoparticles (Au NPs) in time-varying electromagnetic fields cell membrane permeabilization, estimating the influence of the presence of Au NPs on transmembrane potential and on the pore opening dynamics. To account for variability and uncertainty about geometries and relative placement and aggregations of the Au NPs, three different NP configurations were considered: spherical Au NPs equally spaced around the cell; cubic Au NPs, for accounting for the possible edge effect, equally spaced around the cell; and spherical Au NPs grouped in clusters. The results show that the combined use of Au NPs and a time-varying magnetic field can significantly improve the permeabilization of cell membranes. The variability of NPs’ geometries and configurations in proximity of the cell membrane showed to have a strong influence on the pore opening mechanism. The study offers a better comprehension of the mechanisms, still not completely understood, underlying cell membrane permeabilization by time-varying magnetic fields.