The use of supplemental STR loci for resolving difficult family relationship cases

Abstract We routinely employ secondary panels of STR loci in conjunction with the Identifiler multi-locus PCR system to help resolve problematic biological relationship tests such as sibships, those involving related alleged fathers or those with genetic inconsistencies. One multiplex (DDCPlex) contains commercially available STR monoplexes, a second contains a 4-locus multiplex made up of "mini-STRs". We present examples whereby DDCPlex and mini-STR loci have proven useful in resolving complex kinship and parentage cases.