Study on alternative methods for the acute oral toxicity in detection of chemicals

Objective To evaluate three alternative methods for LD50 test-Fixed Dose Procedure (FDP),the Acute Toxic Class Method (ATC) and Up and Down Procedure (UDP).Methods Female SD rats (8～12 weeks of age,160~200 g) were used.Three alternative methods from OECD were applied to assess 22 chemicals (10 cosmetic raw materials and 12 raw materials of personal and home care products).The toxicity ranking for tested chemicals was established according to Globally Harmonized System(GSH).The results LD50 test were compared for the consistency and correlation between alternative methods and traditional test.Results For cosmetic raw materials,the concordance rate of the three alternative methods was 80%(8/10);for raw material of personal and home care products,the concordance rates of FDP,ATC and UDP was 91.7%(11/12),75.0%(9/12) and 83.0%(10/12),respectively.The number of animals required in three alternative methods was significantly lower than that in traditional test (P<0.05),but the time required in three alternative methods was significantly higher than that in traditional test (P<0.05 ).Conclusions High consistency and correlation were found between each alternative method and LD50 test.FDP may be more potential when applied to assess acute oral toxicity of cosmetic raw materials. Key words: Toxicity tests; Animal testing alternatives; Cosmetics; Inorganic chemicals; Organic chemicals