Effects of macrophage colony-stimulating factor on the proliferation and the function of kupffer cells

Using flow cytometric methods, the influence of recombinant human macrophage colony-stimulating factor (rhM-CSF) on DNA synthesis, the phagocytosis of microspheres and the uptake of lipopolysaccharide (LPS) in cultured rat Kupffer cells was investigated. Incubation with 10 3 U/ml of rhM-CSF did not increase the S-phase population of Kupffer cells, however, 10 5 U/ml of M-CSF significantly increased that population from 6.26±0.68% to 8.31±0.99%